Methylation
Sequencing

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What we offer

Streamlined workflow
from library prep to analysis powered by Illumina

Targeted but comprehensive
bisulfite conversion followed by target enrichment of methylation sites

Breadth of results
traditional and emerging epigenetic regions are covered by targeting 3.3 million CpGs

Our process

The Bisulfite sequencing technique is a combination of bisulfite conversion and subsequent sequencing. This technology is the fundamental tool for genome-wide DNA methylation analysis.

1

Library Preparation

The library prep is performed with:

  • TruSeq Methyl Capture EPIC Library Prep Kit

2

Sequencing

At MLLSEQ, sequencing is performed using the Illumina sequencing by synthesis method on the latest generation of sequencing devices, the NovaSeq 6000.

3

Data are analzyed within the Illumina BaseSpace Sequencing hub:

  • BaseSpace MethylSeq App
  • BaseSpace MethylKit App

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