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Single-cell sequencing examines the sequence information from individual cells, allowing a higher resolution of cellular differences and determination of the characteristics of individual cells.
What we offer
At MLLSEQ, we have the sequence capacity and experience to support you with our expertise. We offer sequencing and data preprocessing of your ready-to-load libraries.
At MLLSEQ, sequencing is performed using the Illumina sequencing by synthesis method on the latest generation of sequencing devices, the NovaSeq 6000. Depending on the library properties different sequencing settings are available.
For 10x genomics single cell gene expression and single cell ATAC libraries the obtained sequencing data is preprocessed with the Cell Ranger/Cell Ranger ATAC pipeline:
- mkfastq: Demultiplexing of the raw sequencing data to obtain sample-specific FASTQ files
- count: Alignment, filtering, Barcode counting, UMI counting, etc.